Try to Apply T Cell Receptor Repertoire Diversity Detection for Acquired Immune Deficiency Syndrome Research of Traditional Chinese Medicine

Abstract

A new approach will be carried out to make clear the mechanism of promotion immune system function with TCM treatment on AIDS and provide a parameter of clinical therapeutic effects. DNA was extracted with periphery blood from forty AIDS patients before and after treatment, and multiple primers PCR technique and capillary electrophoresis were used to detect T cell receptor beta repertoire diversity. Meanwhile, seven control subjects were measured repeatedly to define the threshold. Sets of typical patterns for positive and negative were shown respectively from the scanning map angle. Moreover, positive detection rates of the forty AIDS patients were calculated for different T cell receptor gene segments. To some degree, this study is suggestive of it how TCM treatment effects on AIDS. However, the reliability needs further verification.